关键词: 高原缺氧, 血常规, 血气, 血生化, 氧化应激

Abstract: Objective To investigate the effects of simulated plateau acute decompression hypoxia on blood indexes in rats. Method Forty healthy male Wistar rats were randomly divided into normoxia control group, hypoxia 10 , 20 , 30 h group, 10 rats in each group. were put into hypobaric hypoxia treatment to simulate the low-pressure and hypoxic environment at 8 000 m altitude. Normal rats were kept at 1 500 m above sea level, the other 3 groups stayed at simulated altitude of 8 000 m for 10 , 20 and 30 h respectively by large low pressure and low oxygen animal laboratory. Check blood sugar, blood gas, blood routine, and blood biochemistry. Observe the effects of different hypoxia time on hematological indexes of rats. Result With the prolongation of hypoxia time, the blood glucose of the rats also increased; According to the blood gas index, the partial pressure of carbon dioxide ( PaCO2 ) , partial pressure of oxygen ( PaO2 ) , pulmonary oxygen saturation ( SaO2 ) gradually decrease with the prolongation of hypoxia; Blood routine result showed that white blood cells (WBC) , red blood cell count (RBC) and platelet ( PLT) in rat arterial blood increased significantly with the prolongation of hypoxia; blood biochemical result showed that albumin ( ALB) , serum urea nitrogen ( BUN) were abnormally elevated. creatine kinase (CK) , and creatine kinase isoenzyme ( CK-MB) were significantly decreased (P < 0. 01 or P < 0. 05 ) . Conclusion The hematological parameters of rats in different periods of simulated plateau hypoxia were changed, which seriously affected the normal physiological activities of rats.

Key words: high altitude hypoxia, blood routine, spirit of life, blood biochemistry, oxidative stress