D-半乳糖诱导青年树鼩卵巢衰老

doi:10. 3969 / j. issn. 1006-6179. 2023. 05. 003

实验动物科学 ›› 2023, Vol. 40 ›› Issue (5): 12-18.DOI: 10. 3969 / j. issn. 1006-6179. 2023. 05. 003

D-半乳糖诱导青年树鼩卵巢衰老

(中国人民解放军联勤保障部队第 920 医院基础医学实验室,云南省细胞治疗技术转化医学重点实验室,干细胞与免疫细胞生物医药技术国家地方联合工程实验室,昆明 650032)

收稿日期:2023-03-06 出版日期:2023-10-28 发布日期:2023-10-30
通讯作者: 潘兴华( 1963—) ,男,主任医师,研究方向:干细胞抗衰老研究. E-mail:xinghuapan@ aliyuan. com
作者简介:田川( 1994—) ,男,研究方向:干细胞抗衰老研究. E-mail:2896981795@ qq. com
基金资助:
全军实验动物专项重点项目( SYDW[ 2020] 19) ;军队实验动物专项科研课题青年基金( SYDW_KY[ 2021] 18)

D-galactose Induced Young Tree Shrews Ovarian Senescence

( the Basic Medical Laboratory of 920th Hospital of Joint Logistics Support Force of PLA, the Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunnan Province, the Integrated Engineering Laboratory of Cell Biological Medicine of State and Regions, Kunming 650032, China)

Received:2023-03-06 Online:2023-10-28 Published:2023-10-30

摘要/Abstract

摘要： 目的通过 D-半乳糖诱导法建立树鼩衰老模型,为卵巢衰老的研究提供模型参考方案。方法 ( 1) 筛选青年树鼩 8 只,随机分为对照组( n = 4)与模型组( n = 4) 。 (2)模型组按 8 g / kg 的剂量颈背部皮下注射 D-半乳糖 5 个月,采集卵巢,液氮冻存以及 4%多聚甲醛固定。 (3) HE 染色观测卵巢组织结构;免疫组织化学染色观测衰老相关标志分子蛋白表达;Ki67 染色观测增殖活性;Tunel 染色观测细胞凋亡。结果 ( 1) 对照组树鼩卵巢组织髓质与间质界限明显,细胞排列整齐,可见原始、初级、次级和成熟卵泡,有少量闭锁卵泡;模型组树鼩卵巢组织结构散乱,局部只见原始、初级卵泡,有大量闭锁卵泡。 ( 2) 模型组衰老相关标志分子 P53、P21、P16 蛋白表达水平明显高于对照组。 (3)模型组细胞增殖活性显著低于对照组。 (4)模型组卵巢细胞凋亡率显著高于对照组。结论 D-半乳糖可诱导卵巢组织结构散乱,卵泡闭锁,使细胞增殖活性减弱,凋亡率增加。

关键词: 树鼩, 卵巢衰老, 卵泡, 细胞凋亡, 衰老标志分子

Abstract: Objective Establishing the aging model of tree shrew induced by D-galactose and providing a model reference scheme for the study of ovarian senescence. Method (1) eight young tree shrews were selected and randomly divided into control group ( n = 4) and model group ( n = 4) . (2) the model group was subcutaneously injected with D-galactose on the back of neck according to the dose of 8 g / kg for 5 months, and the ovaries were collected, frozen in liquid nitrogen and fixed with 4% paraformaldehyde. (3) the tissue structure of ovary was observed by HE staining, the expression of aging-related molecular proteins was observed by immunohistochemical staining, cell proliferation activity was observed by Ki67 staining, and apoptosis was observed by Tunel staining. Result (1) the boundary between medulla and stroma in the ovarian tissue of tree shrew in the control group was obvious, and the cells were arranged neatly, showing primitive, primary, secondary and mature follicles with a small number of atretic follicles, while in the model group, the ovarian tissue structure of tree shrew was scattered, only primitive and primary follicles were seen locally, and there were a large number of atretic follicles. (2) the expression level of aging-related marker molecules p53, P21 and P16 in the model group was significantly higher than that in the control group. (3) the activity of cell proliferation in the model group was significantly lower than that in the control group. (4) the apoptosis rate of ovarian cells in the model group was significantly higher than that in the control group. Conclusion D-galactose can make ovarian tissue structure scattered, follicle atresia, weaken cell proliferation activity, and increase apoptosis rate.

Key words: Tree shrew, ovarian senescence, follicle, apoptosis, aging marker molecule

中图分类号:

Q95-3

田川, 叶丽, 赵晓娟, 朱向情, 赵晶, 阮光萍, 潘兴华. D-半乳糖诱导青年树鼩卵巢衰老[J]. 实验动物科学, 2023, 40(5): 12-18.

TIAN Chuan, YE Li, ZHAO Xiaojuan, ZHU Xiangqing, ZHAO Jing, RUAN Guangping, PAN Xinghua. D-galactose Induced Young Tree Shrews Ovarian Senescence[J]. Laboratory Animal Science, 2023, 40(5): 12-18.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: http://www.sydwkx.com/CN/10. 3969 / j. issn. 1006-6179. 2023. 05. 003

http://www.sydwkx.com/CN/Y2023/V40/I5/12

http://m.chaoxing.com/mqk/read_38502727e7500f266a209ee00091c89c73dc1fda737368f71921b0a3ea255101fc1cf1fbb4666ae6f31e1bb821844c4985b96c726c9c142ce3dee8926f91a638d2b565681abb33b2c516f82d245def59?from=screen

[1]	李夏莹, 田永路, 韦玉生. 树鼩微生物感染研究进展[J]. 实验动物科学, 2023, 40(6): 99-103.
[2]	田永路, 李娜, 田祯, 曾申明, 朱德生. 热应激对 ICR 雌性小鼠超数排卵及卵泡发育的影响[J]. 实验动物科学, 2021, 38(5): 4-.
[3]	巴依尔才次克, 王彦梅, 朱艳萍. 间充质干细胞移植改善脑室周围白质软化损伤作用的研究 [J]. 实验动物科学, 2021, 38(2): 22-.
[4]	田川, 杨再玲, 朱向情, 李晔, 吕冠柯. 猕猴从幼年到老年的卵巢组织学变化[J]. 实验动物科学, 2020, 37(06): 37-.
[5]	王静，洪炳哲，张习敬，任海勤，葛鲁敏. PTS预处理对大鼠心肌缺血再灌注损伤保护效果及机制研究[J]. 实验动物科学, 2020, 37(01): 27-.
[6]	王文广，匡德宣，仝品芬，李娜，陆彩霞，罕园园，孙晓梅，代解杰. 树鼩的标准化研究与应用进展[J]. 实验动物科学, 2020, 37(01): 74-.
[7]	宋庆凯，尹博文，陈玲霞，李晓飞，苗雨润，代解杰，孙晓梅. 树鼩胸主动脉血管内皮细胞的分离培养与鉴定[J]. 实验动物科学, 2018, 35(06): 62-.
[8]	苗雨润，李娜，匡德宣，宋庆凯，袁圆，王璇，张志成，陆彩霞. 不同诱导条件对树鼩骨髓间充质干细胞向成骨细胞分化的影响[J]. 实验动物科学, 2018, 35(05): 46-.
[9]	王晶晶，张杰，卢静. 内质网应激对心肌梗死后心力衰竭大鼠心肌组织的作用[J]. 实验动物科学, 2018, 35(02): 6-.
[10]	陈玲霞，尹博文，苗雨润，宋庆凯，李晓飞，代解杰. 树鼩粪便中病毒三种浓缩方法的效果比较[J]. 实验动物科学, 2018, 35(02): 38-.
[11]	李昕潼, 陶玉芬, 刘建生, 代解杰, 刘红旗. 树鼩细胞免疫表型分析研究的展望[J]. 实验动物科学, 2017, 34(04): 74-78.
[12]	尹博文, 陈玲霞, 李振宇, 李晓飞, 宋庆凯, 苗雨润, 孙晓梅. 树鼩小肠上皮细胞的体外分离培养与鉴定[J]. 实验动物科学, 2017, 34(02): 11-15.
[13]	苗雨润, 宋庆凯, 陈玲霞, 尹博文, 李晓飞, 代解杰. 树鼩角膜内皮细胞体外原代培养方法的建立[J]. 实验动物科学, 2016, 33(05): 1-8.
[14]	罕园园, 孙晓梅, 匡德宣, 陆彩霞, 仝品芬, 王文广, 李娜, 余波, 代解杰. 树鼩脂肪转录组SNP 位点发掘及其功能注释[J]. 实验动物科学, 2016, 33(03): 13-19.
[15]	谢明仁, 张璟, 俞发荣. 低频电刺激对脏器中激素含量的影响[J]. 实验动物科学, 2015, 32(04): 26-30.

D-半乳糖诱导青年树鼩卵巢衰老

D-galactose Induced Young Tree Shrews Ovarian Senescence

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics