关键词:

关键词: 皮肤致敏, 三硝基苯磺酸, 实验性结肠炎, 多功能液相芯片, 小鼠

Abstract:

Abstract: Objective Whether skin presensitization is necessary in experimental colitis model induced by trinitrobenzenesulfonic acid ( TNBS ) has not been determined, and the effect of skin sensitization on it is investigated. Method BALB / c mice were randomly divided into normal control group ( NC group ) , skin presensitization group ( SP group) , and non-skin presensitization group ( NP group) , 5 per group. The success of the two groups of models and the Luminex multifunctional liquid chip analysis system was used to evaluate the differential expression levels of BAFF / BLys / TNFSF13B、 TNF-α、 ICAM-1 / CD54 and TIMP-1 inflammatory cytokines in serum. Result Compared with the NC group, there was no significant difference in body weight between SP group and NP group from skin presensitization to before enema; After enema, the body weight of SP group was significantly lower than NP group ( P<0. 05 ) . Compared with the NC group, the spleen weight ( P<0. 01) and spleen index ( P<0. 05) of the NP group were significantly reduced; the scores of disease activity index in SP group and NP group were significantly higher than the NC group ( P <0. 05) ; the colon macroscopic damage index ( CMDI) in the SP group increased significantly ( P<0. 05) ; the SP group had a significant increase in histopathological score ( P<0. 05) . Serological detection of inflammatory cytokines, the result of BAFF / BLys /TNFSF13B showed that compared with the NC group, the SP group was increased significantly ( P<0. 01) , but there was no significant difference between the SP group and the NP group. Conclusion Both the SP group and the NP group can successfully establish model of acute experimental colitis, but the benefit of acute experimental colitis established by skin presensitization has a better modeling effect.

Key words:

Key words: skin presensitization, trinitrobenzenesulfonic acid, experimental colitis, multifunctional liquid chip, mice