关键词:  , LncRNA, Snhg5, 心肌细胞, 转基因小鼠, 心脏重塑 

Abstract: Objective To establish a transgenic mouse with cardiomyocyte-specific overexpression of Snhg5.Method A construct that directed the expression of Snhg5 to cardiomyocytes was made. The linearized constructs were introduced into the fertilized eggs by microinjection to obtain the transgenic mice. The integration of Snhg5 gene was detected by PCR. The tissue-specific expression of Snhg5 was confirmed by Real-time PCR. Cardiac phenotypic analysis in control and transgenic mice were performed at the gross morphological, histological and molecular levels. Result A transgenic vector that specifically overexpressing Snhg5 in cardiomyocytes was successfully constructed, and 4 Snhg5 transgenic founder mice were successfully obtained by microinjection of fertilized eggs. Detecting the expression level of Snhg5 in different tissues showed that Snhg5 was upregulated specifically in cardiac tissue. Further phenotypic analysis demonstrated that cardiomyocyte-specific Snhg5 transgenic mice were more prone to pathological cardiac remodeling under isoproterenol ( ISO ) stimulation compared to control mice. Conclusion A cardiomyocyte-specific Snhg5 transgenic mouse was generated successfully, providing an ideal mouse model for studying the function of Snhg5 in cardiac tissue and the mechanism underlying the pathological cardiac remodeling.

Key words:

LncRNA, Snhg5, cardiomyocyte, transgenic mice, cardiac remodeling