Abstract: Objective　To analyze the genetic quality of ICR mouse population in the National Rodent Laboratory Animal Resources Database, this study was performed.Methods　The genomic DNA of 30 mice was extracted. Based on the group standard “Laboratory animal-Method for microsateltlie markers of laboratory mice and rats”, 20 microsatellite loci distributed on all chromosomes of mice were selected for PCR amplification. The amplified products were sequenced and analyzed. In addition, the allele numbers, heterozygosity, and polymorphism information content were also calculated. Results　The average number of alleles detected at 20 microsatellite loci in ICR mice was 4.15, with an average heterozygosity of 0.502 and an average polymorphism information content of 0.456.Conclusion　The genetic diversity of the ICR mouse population in the National Rodent Laboratory Animal Resources Center was relatively ideal, meeting the genetic quality requirements of closed colony in national standards.

Key words: ICR mice, genetic quality, microsatellite DNA, closed colony

摘要： 目的　利用微卫星检测法对国家啮齿类实验动物资源库的ICR小鼠种群的遗传质量进行检测分析。方法 提取30只小鼠的基因组DNA,根据团体标准《实验动物 小鼠、大鼠微卫星DNA标记检测方法》,选用覆盖小鼠所 有染色体的20对微卫星位点的引物进行PCR扩增,对扩增产物进行测序分析,计算等位基因数、杂合度和多态信 息含量等参数。结果　ICR小鼠的20个微卫星位点的平均等位基因数为4.15个,平均杂合度为0.502,平均多态 信息含量为0.456。结论　国家啮齿类实验动物资源库保藏的ICR小鼠种群的遗传多样性较好,符合国家标准中 规定的封闭群小鼠遗传质量要求。

关键词: ICR小鼠, 遗传质量, 微卫星DNA, 封闭群