Abstract: Objective　In this study, metagenomic high-throughput sequencing of respiratory tract and intestinal samples from laboratory dogs and companion dogs was performed to obtain the composition and function of their respective microbiota. Methods　Metagenomic high-throughput sequencing was performed on respiratory tract and intestinal samples from 13 Laboratory dogs and 31 companion dogs and relevant analyses were conducted.Results　There were significant differences in species composition and function between laboratory dogs and companion dogs in terms of respiratory tract and intestinal microbiota.Conclusion　The differences in the composition and function of respiratory tract and intestinal microbiota between laboratory dogs and companion dogs are revealed, and provide microbiome basis for the optimization of experimental animal models and pet health management.

Key words: metagenomic sequencing, microbiota composition, functional analysis of microbiota 

摘要： 目的　本研究通过对实验犬与宠物犬呼吸道和肠道样本进行宏基因组高通量测序,以期获得各自菌群组成 及功能特征。方法　对13只实验犬和31只宠物犬呼吸道和肠道样本进行宏基因组高通量测序并进行相关分析。 结果　实验犬与宠物犬呼吸道及肠道菌群在物种组成和功能特征上存在显著差异。结论　揭示了实验犬与宠物 犬呼吸道和肠道菌群组成及功能差异,为实验动物模型优化及宠物健康管理提供微生物组学依据。

关键词: 宏基因组测序, 菌群组成, 菌群功能分析