Laboratory Animal Science ›› 2025, Vol. 42 ›› Issue (5): 7-15.DOI: 10.3969/ j. issn.1006-6179.2025.05.002

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 High-throughput Sequencing Analysis of the Respiratory and Gut Microbiota in Sheep

  

  1. (1.Collage of Veterinary Medicine,Jilin University, Changchun 130062,China) (2. College of Animal Science, Jilin University, Changchun 130062, China)
  • Received:2025-04-22 Online:2025-10-28 Published:2025-11-05

高通量测序分析绵羊呼吸道及肠道菌群

  

  1.  (1.吉林大学动物医学学院,长春 130062)(2.吉林大学动物科学学院,长春 130062)
  • 通讯作者: 杜崇涛(1979—),男,副教授,研究方向为实验动物微生物学。E-mail:duct@jlu.edu.cn。
  • 作者简介:周 昂(2000—),男,硕士,研究方向为兽医学。E-mail:13290348002@163.com。 黄荣磊(1998—) ,男,博士,研究方向为实验动物微生物学。 E-mail:hrl17835714615@163.cm。
  • 基金资助:
    国家重点研发计划(2022YFF0710504);吉林省科技发展计划项目(20230505032ZP)。

Abstract: Objective The study employs microbial diversity composition profiling and metagenomic sequencing to investigate the compositional characteristics and functional analysis of respiratory and intestinal microbiota in sheep from Jilin Province, China, thereby providing foundational data for the establishment of experimental sheep models.Methods Microbiota diversity composition profiles and metagenomic sequencing were performed on respiratory and intestinal samples from 200 sheep.Results The study revealed the composition of the respiratory and intestinal microbiota of sheep, as well as their respective characteristics in terms of microbiota structure and functional potential.Conclusion The findings provide insights into the respiratory and intestinal microbiota of sheep and their functions, offering a solid theoretical foundation for establishing detection standards for experimental sheep.

Key words: microbiomics, microbiota composition, microbiota diversity

摘要: 目的 探究吉林省绵羊呼吸道和肠道菌群的组成特征及功能分析,并为实验羊模型的建立提供基础数据。 方法 对200只绵羊的呼吸道和肠道样本进行菌群多样性组成谱和宏基因组测序分析。结果 揭示了绵羊的呼 吸道及肠道菌群组成,及两者在菌群结构和功能方面的特点。结论 揭示了绵羊呼吸道和肠道菌群及功能,为实 验羊检测标准的建立提供坚实的理论依据。

关键词: 微生物组学, 菌群组成, 菌群多样性

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