Abstract: Objective　To understand the drug sensitivity and resistance genes carried by Salmonella from tree shrews.Methods　Salmonella was isolated and purified from the intestinal contents of tree shrews, followed by biochemical analysis and K-B drug sensitivity test for bacterial identification. PCR technology was used to detect the resistance genes of the strains.Results　The pathogenic identification result confirmed that the isolated strains were Salmonella; The result of the drug sensitivity test showed that the isolated strains were resistant to six antibiotics, including penicillin, doxycycline, minocycline, erythromycin,tetracycline and benzylpenicillin,while five resistance genes, including quinolone qnrS , tetracycline tetA,tetB and β-lactam TEM,SHV were detected.Conclusion　The Salmonella isolated from Tree shrews in this study carries multiple resistance genes and is resistant to some antibiotics.

Key words: tree shrew, Salmonella, drug resistance

摘要： 目的　了解树鼩源沙门菌的药物敏感性及所携带耐药基因的情况。方法　通过对树鼩肠道内容物中 沙门菌的分离纯化、生化分析和K-B药敏实验对细菌进行鉴定,并应用PCR技术对所分离到的菌株进行耐 药基因的检测。结果　经病原学鉴定,确定所分离的菌株为沙门菌。药敏实验结果表明,分离株对青霉素、 强力霉素、米诺环素、红霉素、四环素、苯唑西林6种抗生素产生耐药性,同时检测出喹诺酮qnrS,四环素tetA、 tetB,β-内酰胺类TEM、SHV 5种耐药基因。结论　本研究所分离出的树鼩源沙门菌,携带有多种耐药基因, 并对部分抗生素耐药。

关键词: 树鼩, 沙门菌, 耐药性