Abstract: Objective　To reveal the characteristics of spontaneous renal calcification during the development of naked mole rats, and to provide a valuable animal model for the study of renal calcification and prevention strategies for chronic kidney disease.Methods　Urine and serum were collected from Newborn, 12-month-old, and 120-month-old naked mole-rats for routine urinary and renal function tests; renal pathology was observed using HE staining, alizarin red staining, Von Kossa staining, and vWF staining; and metabolomics analysis was performed on kidney tissues of 12-month-old naked mole rats.Results　There were no significant differences in kidney function between 120-month old and 12-month-old NMRs. The vascular morphology was normal in NMRs with different ages and the calcification. Lipids and lipid-like substances, organic acids and their derivatives, undefined substances, organic heterocyclic compounds, and organic oxygen compounds accounted for 82.668% of the total metabolites in the kidneys of adult NMRs. Conclusion　By systematically exploring the functional characteristics of the kidneys of NMRs, especially the phenomenon of kidney calcification in NMRs as they age, it is revealed that spontaneous renal calcification of NMRs maintains a normal relationship with their kidney function.

摘要： 目的　揭示裸鼹鼠在发育过程中自发性肾钙化的特性,为肾钙化-慢性肾病的防治策略研究提供有价值的动 物模型。方法　采集新生、12月龄、120月龄裸鼹鼠尿液和血清用于尿常规和肾功能检测;利用HE染色、茜素红染 色、Von Kossa染色和vWF染色进行肾病理观察;并对12月龄裸鼹鼠肾组织进行代谢组学分析。结果　120月龄与 12月龄裸鼹鼠肾功能无显著差异,血管形态均较正常,钙化显著。成年裸鼹鼠肾代谢物中脂类及脂类样物质、有机 酸及其衍生物、未定义物、有机杂环化合物和有机氧化合物占总代谢物的82.668%。结论　通过系统探究裸鼹鼠肾 的功能特性,尤其是随着年龄增长裸鼹鼠存在肾钙化现象,揭示裸鼹鼠肾自发性钙化与其肾功能保持正常的关系。

关键词: 裸鼹鼠, 肾, 钙化, 慢性肾病, 动物模型