关键词: 封闭群动物, 近交系动物, 免疫缺陷动物, 胚胎数量, 产仔率 

Abstract: Objective Effects of different numbers of embryos transferred in mice of different genetic backgrounds on the litter rate. Method The 2-cell embryos were transplanted into pseudopregnant mice by fallopian tube transfer technique. Then, Statistical analysis was performed on the litter size of pregnant animals. Result Compared with the closed colony, the transplant efficiency of the inbred strain animals is lower, the average litter rate is 37. 5%, and the closed colony is 45%. Immunodeficient animals have higher requirements for the number of embryos to be transplanted, and mice with 11-15 cells have the highest litter rate, 63. 5%; In this study, mice with different genetic backgrounds have different mean litter rate: C57BL / 6J ( 49. 8%) > KM ( 45. 8%) > ICR (44. 1%) > NOD-SCID (35. 6%) ; > BAL B / c -nu ( 29. 3%) . In this study, it was found that mice of different genetic backgrounds required different numbers of embryos to achieve higher litter rate. ICR, C57 and NOD-SCID mice need to transfer 11-15 embryos, KM mice need to transfer 16-30 embryos; BALB / c-nu mice need to transfer ≤10 embryos. Conclusion Tubal transplantation differs in mice with different genetic backgrounds. The litter rate of mice of the same genetic background varies with the number of transferred embryos.

Key words: closed colony, inbred strain animals, immunodeficient animals, number of embryos, litter rate